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Transduced adult stem cells into in
畢業(yè)論文
| 全部作者： | 楊川 程樺 李焱 劉丹 王川 嚴勵 傅祖植 |
| 第1作者單位： | 中山大學附屬第2醫(yī)院內(nèi)分泌科 |
| 論文摘要： | The promising results in replacement of the beta-cell mass have been accomplished by beta-cells implanting. However, the demand for beta-cells is far greater than the supply. The transdifferentiation of stem cells into insulin-secreting cells could potentially provide a new help for beta-cell supplying. To determine whether the myoblast cell line C2C12 could be induced to transdifferentiate into insulin-secreting cells by pancreatic-duodenum homeobox-1 (PDX-1). A myoblast cell line (C2C12) was transfected by the PDX-1 constructing to overexpress PDX-1 protein and then treated with various concentrations of glucagon-like peptide-1 (GLP-1). The Expression of PDX-1 and insulin mRNA was determined by in situ hybridization and the intracellular insulin was determined by immunocytochemistry. The result shows that the PDX-1 transfected cells expressed more insulin and secreted more insulin than the vector transfected cells. We conclude that PDX-1 and GLP-1 might induce myoblasts to transdifferentiate into insulin-secreting cells. |
| 關鍵詞： | GLP-1, glucagon-like peptide 1; ES, embryonic stem; DTZ 遠程下載 論文(免費PDF論文全文) |
| 發(fā)表日期： | 2005年12月12日 |
| 同行評議： | 目前成體干細胞的可塑性時最為引人注目的，本文的研究進1步進行了檢測，將為糖尿病的治療提供誘人的臨床前景。值得發(fā)表。 |
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| 注：同行評議是由特聘的同行專家給出的評審意見，綜合評價是綜合專家對論文各要素的評議得出的數(shù)值，以1至5顆星顯示。 | |
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